The Gentier 96E/96R Real-Time PCR Machine is a cutting-edge RT PCR analyzer designed for high-precision and efficient thermal cycling. Ideal for a wide range of applications in research and diagnostics, this machine offers robust performance and advanced features for accurate real-time PCR analysis.

Thermal Block Specifications:

• Sample Capacity: 96 wells
• Reaction Volume: 0-100 μL
• Compatible Consumables: 0.2ml 96-well plates (unskirted), 0.2ml 8-strip tubes, 0.2ml PCR single tubes (Optical flat cap, clear, frosted, white tube)
• Temperature Range: 0°C-100°C
• Heating/Cooling Method: Peltier
• Maximum Heating Rate: 6.1°C/sec
• Average Heating Rate: 4.5°C/sec
• Maximum Cooling Rate: 5.0°C/sec
• Average Cooling Rate: 2.8°C/sec
• Temperature Accuracy: ±0.1℃
• Temperature Uniformity: ±0.1℃
• Gradient Range: 1°C-40°C
• Gradient Block: 12 rows
• Special Temperature Protocols: Gradient PCR, Long PCR, Touch Down PCR

Heat Lid:

• Temperature Range: Room Temperature–110°C

Optical System:

• Excitation Source: 6 LEDs
• Detector: Photodiode
• Detection Position: Top excitation and scan
• Detection Method: 6 channels scanning simultaneously, eliminating edge effects
• Detection Time: 7 seconds for 96 wells across all channels
• Excitation/Emission Wavelengths (nm):
  • Channel 1: 465 / 510 (FAM, SYBR Green I, SYTO9, EvaGreen, LC Green)
  • Channel 2: 527 / 563 (HEX, VIC, TET, JOE)
  • Channel 3: 580 / 616 (ROX, Texas Red)
  • Channel 4: 632 / 664 (Cy5)
  • Channel 5: 680 / 730 (Alexa Fluor680)
  • Channel 6: 465 / 616 (FRET)
• Probe Compatibility: Taqman Probe, Molecular Beacons Probe, Scorpion Probe, FRET
• Multiplexing Capability: Up to 6 targets
• Fluorescence Linearity: r ≥ 0.990
• Fluorescence Dynamic Range: Adjustable

Performance:

• Sample Linearity: /r/ ≥ 0.999
• Sample Repeatability: Ct value CV ≤ 0.5%
• Sample Dynamic Range: 1-10^10 copies

Software Functions:

• Data Analysis Modes: Qualitative analysis, Absolute quantification, Relative quantification, Genotyping analysis, Endpoint analysis, Melt curve analysis, High Resolution Melting
• Control Modes: 10.4" touch screen, PC direct control, WLAN control (up to 10 units on one PC)
• Sample Drawer Control: Touch screen command
• Data Storage: Upload and download via USB, with storage capacity for up to 1,000 results
• Power Failure Protection: Automatically resumes experiments after power restoration without waiting for PC software
• Customizable Reports: Templates available for personalized report generation
• Administration Management: Allows setting of function limits for users
• Transport Locker: Automatically detects transport lock
• Fault Management: Includes fault reporting, analysis, and solution instructions
• LIS Connection: Outputs data in CSV, Excel, TXT formats, with an open port for LIS integration

Additional Specifications:

• Operating System: Windows 7, Windows 10
• Communication Ports: 1 Ethernet and 3 USB
• Footprint (WxDxH): 355mm x 480mm x 485mm
• Weight: 30kg
• Power Usage: AC 100 to 125 V / 200 to 240 V (50/60 Hz), 900VA
• Work Environment: Temperature 10°C~30°C, Humidity 20%~85%

This real-time PCR machine offers unparalleled precision, user-friendly operation, and comprehensive data management, making it an essential tool for modern laboratories.